FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n02_P3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n02_P3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37219137
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC59200.011.24920211
TCAGGTA69400.09.8238737
CGTGACT29500.09.4642932
GTACTCG64850.09.3036214
GGTAGAA74800.08.75269710
GAATCGA67700.08.61267954
TAGAACA77700.08.5421412
CAGGTAG77600.08.4083158
GGAAAAT137400.08.3614621
TACTCGA72050.08.3114125
GAATTGA94850.08.0224414
AAAATCC98850.07.85723263
CACTCGT52250.07.836132535
CTCGAAT40700.07.80411151
AGTACTC85850.07.6571953
AATCGAA68600.07.6136185
GGGAAAT83400.07.40087841
AATTGAC57100.07.293955
AGATCAG137600.07.28062734
GAAAAAT218000.07.17136341
Produced by FastQC (version 0.11.5)