FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n02_P3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n02_P3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30077997
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG21300.013.52054616
CGTGACT27600.013.4606242
GTAGAAC51900.012.00781511
TCAGGTA58700.011.0779027
TGTAGGG28150.010.86983418
GTGTAGG28400.010.85340717
GCGTGAC39900.010.5575261
TCGTGTA30250.010.18964515
GGTAGAA64700.09.91040610
GTACTCG55600.09.5960794
CAGGTAG67050.09.5299828
TAGAACA66350.09.42685912
GGAAAAT112300.09.14699651
GTAGATC31300.09.12807632
GAGTGTA27900.09.11157328
TACTCGA59600.08.9520475
GTGACTC46050.08.848493
CTATAAG35950.08.6417254
AATTGAC44750.08.6024735
TATAAGG37950.08.6015445
Produced by FastQC (version 0.11.5)