FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n02_P3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n02_P3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33617209
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC57200.011.99648511
CGTGACT27400.011.5870172
TCAGGTA68650.010.6522217
CAGGTAG74150.09.4670858
GGTAGAA74150.09.40588110
GTACTCG70350.09.28031354
TAGAACA75150.09.2511412
GCGTGAC40200.09.1341811
GAATCGA58100.08.9508564
TACTCGA73150.08.6788765
GGAAAAT140650.08.6489241
AGATCAG122650.08.3883664
AGTACTC81000.08.3658533
AGAACGG233000.08.3384173
AAAATCC100350.08.1211953
AGGTAGA89900.08.0085059
GAATTGA84850.07.9597264
GCCATGT204750.07.889816817
AATCGAA62500.07.85245045
AATTGAC52700.07.8175245
Produced by FastQC (version 0.11.5)