FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n02_P3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n02_P3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30295967
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28500.015.3268432
GCGTGAC42750.010.90768051
GTAGAAC41900.010.20213811
GTACTCG65100.09.8904234
GTGACTC50050.09.5808793
TACTCGA67000.09.542735
GGAAAAT125500.09.4235331
CTAGCAC50350.09.38393714
GCTCTAG55550.08.95075811
TCAGGTA52000.08.7847
AGTACTC78950.08.6971623
AAAATCC91400.08.54698853
TGACTCT61100.08.2166264
GAATCGA49000.08.0862364
GGACTTA42950.08.01974
GGTAGAA55950.08.00209910
CTCGAAT28850.07.8863081
CGTTACG4853.3799188E-77.88541243
AATCGAA57800.07.7119915
AATTGAC51050.07.67330845
Produced by FastQC (version 0.11.5)