FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n02_P3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n02_P3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28906120
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24150.012.5865482
GTAGAAC43450.010.77023911
GCGTGAC34500.09.8593931
CTCGAAT29350.09.67062951
GAATCGA50450.09.5494084
TCAGGTA51900.09.5378227
GTACTCG67900.08.8524884
AATCGAA60400.08.4980985
TAGAACA55700.08.40182112
GGTAGAA58250.08.38138210
GGAAAAT124100.08.313541
TACTCGA72500.08.1666065
CAGGTAG59300.08.1575058
GTGACTC41900.07.95191143
AGTACTC80800.07.8849493
GGACTTA42050.07.8700084
AAAATCC87600.07.83825833
CCGTATG23850.07.546035345
GACTTAT44000.07.5212245
GAATTGA71550.07.42550854
Produced by FastQC (version 0.11.5)