FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n02_P3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n02_P3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29901600
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT33500.018.4162222
GCGTGAC46900.014.6978391
GTGACTC54400.012.208063
GTAGAAC40450.011.12398511
GCTCTAG62400.010.96069111
CTAGCAC61550.010.63729714
TCAGGTA47350.010.4069437
TGACTCT66900.09.9606844
GTACTCG70350.09.6962114
TACTCGA70300.09.446925
AAAATCC95000.09.3367593
GGAAAAT126250.09.1892631
ACTCTGC78850.08.9355336
AGTACTC79050.08.88538553
CTCGAAT30750.08.8643141
CTCTATA67800.08.85929745
GAATTGA74700.08.8000634
CACTCGT65600.08.77920635
TCTAGCA74200.08.67218413
AAATCCT102800.08.6721284
Produced by FastQC (version 0.11.5)