FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n02_P3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n02_P3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29071605
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT30800.015.0596332
GCGTGAC43250.011.4586471
GTACTCG65250.010.5920734
GTGACTC49950.010.321053
TACTCGA69550.010.0990515
GTAGAAC40050.010.05550511
AGTACTC75600.09.7077743
CTCGAAT29300.09.3028181
AAAATCC91850.09.1177493
GCTCTAG58200.09.00712811
GGAAAAT130150.09.0002781
GAATCGA47150.08.7853534
CACTCGA32050.08.639734
CTAGCAC58000.08.57292114
TCAGGTA50650.08.52947
TGACTCT59950.08.3741134
AATCGAA57550.08.3712755
GAATTGA72500.08.1976434
AGAACGG181250.08.0237683
AAATCCT103900.07.99528464
Produced by FastQC (version 0.11.5)