FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16406146
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG9900.034.25817515
CGTCTGA10600.031.78472316
CACACGT13100.027.09408212
CACGTCT12500.026.95689414
ACACGTC15100.023.35669113
ATCGGAA17400.022.721872
TCGGAAG17350.022.5277183
AGCACAC15850.021.82702810
CAGTCAC10450.021.59282127
GATCGGA20450.019.2260591
CGGAAGA20700.019.207484
CCAGTCA12700.019.01109326
GCACACG18250.018.8335311
GAGACCG5650.016.33477831
CTCCAGT18300.015.90665724
ACCGATC5150.015.71775334
AACTCCA21500.015.10968122
CCGAGAC5550.015.04258329
AGCGGTT6600.013.95542611
AGCGCTA1000.00763259313.47959838
Produced by FastQC (version 0.11.5)