FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n01_P3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n01_P3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25056350
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG40400.027.17580215
AGTCACG40950.026.37144328
CGTCTGA44200.024.43270716
CTCGTAT39650.023.32810642
TCGTATG45000.021.00509343
TCGGAAG55550.020.2520453
ATCGGAA56900.019.7719422
GTCACGA56650.019.7381929
CACGTCT58300.019.44934814
CACACGT58700.019.43171712
CGTATGC52100.017.36488244
CAGTCAC64600.017.27424827
TCACGAT65200.016.73574330
TCTCGTA66250.014.708961541
ACACGTC78750.014.68432213
CCAGTCA81400.013.70904626
CGGAAGA87750.013.4102684
ACGATCA85800.013.24206732
CACGATC86450.013.03832631
GATCGGA91350.012.8346761
Produced by FastQC (version 0.11.5)