FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n01_P3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n01_P3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24827192
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG43450.028.01228715
CGTCTGA47950.025.47790316
TCGGAAG56950.021.7691653
ATCGGAA57150.021.6146352
GTCACAC57850.020.68992629
CACGTCT61150.020.45593814
CACACGT63900.019.46985612
CAGTCAC67850.018.23738927
AGTCACA70600.017.1763328
CACACTT71600.016.96799731
CTCGTAT29500.016.78350342
ACACGTC80200.016.10185813
CGGAAGA87900.014.4369074
CCAGTCA84000.014.43641426
TCGTATG32750.014.36255743
TCACACT89400.013.91676630
TCCAGTC89550.013.86852625
ACACTTG87200.013.8551232
GATCGGA93450.013.5090081
ACTCCAG101350.012.98645723
Produced by FastQC (version 0.11.5)