FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n01_P3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n01_P3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37219137
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG77400.030.1715515
CGTCTGA83500.027.69841216
CACACGT98000.024.08186112
CTCGTAT64250.024.06448242
ATCGGAA101750.023.2410432
CACGTCT102800.023.04498914
TCGGAAG103800.022.8683833
TCGTATG65600.021.7857643
CAGTCAC115600.019.83177827
ACACGTC126300.019.71900213
CCAGTCA130800.017.61321626
AGTCACC135900.017.18391228
GATCGGA140700.017.0495931
CGGAAGA144900.016.6924614
CGTATGC83200.016.60894644
TCCAGTC143000.016.220925
TCTCGTA110650.014.78674141
GCACACG164350.014.74302611
ACTCCAG164950.014.6491523
AGCACAC168350.014.59318710
Produced by FastQC (version 0.11.5)