FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n01_P3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n01_P3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30077997
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1649210.548311112605005TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG287500.039.9013915
AGTCACG284250.039.13126428
CGTCTGA291700.039.05025516
ATCGGAA305850.038.063112
TCGGAAG304850.038.054423
CTCGTAT234950.037.4624542
CACACGT308950.037.34948312
CACGTCT309300.037.16908314
GTCACGC304400.036.7927229
TATCTCG274800.036.5395739
TCGTATG229500.036.529143
CACGCCA311850.035.5024731
CGCCAAT309150.035.1942433
CAGTCAC319150.035.11314827
ACGCCAA310150.035.0079932
ACACGTC336250.034.5378913
CCAGTCA325350.034.40938626
CGGAAGA338150.034.3468554
CGTATGC238100.034.2449944
GATCGGA342000.034.176131
Produced by FastQC (version 0.11.5)