FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n01_P3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n01_P3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33617209
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG51350.026.68167315
CGTCTGA56000.024.386316
CTCGTAT53050.022.7396442
TCGTATG54250.022.69349743
GTCACAC71100.019.04899829
CGTATGC69950.017.88929444
ATCGGAA77800.017.843922
CACACGT79800.017.78942912
TCGGAAG78800.017.5886823
CACGTCT80600.017.52914814
CAGTCAC84900.016.27056727
AGTCACA89950.015.131906528
ACACGTC102650.014.42123713
AGTGATC91850.014.25671436
TCTCGTA89550.014.0491441
CACAGTG97250.013.55683833
CCAGTCA102500.013.36712526
ACACAGT101750.013.06769432
TCCAGTC107600.012.77559925
GATCGGA112800.012.4085811
Produced by FastQC (version 0.11.5)