FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n01_P3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n01_P3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30295967
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42200.024.6833515
CGTCTGA43800.023.52555516
AGTCACT57800.017.6714828
CTCGTAT38350.017.37076442
ATCGGAA62050.016.9702552
TCGGAAG63350.016.870383
CACGTCT65200.016.49361614
CACACGT67950.015.75988612
TCGTATG41800.015.560575543
ACTGACC65400.015.48048932
CAGTCAC72750.014.10198727
GTCACTG77000.013.49900929
ACACGTC87300.012.91097613
CACTGAC82800.012.52617231
CGTATGC49650.012.32959144
AATCTCG71950.011.9476439
CCAGTCA88850.011.54670426
CGGAAGA99400.011.2725154
GATCGGA97800.010.9755491
GACCAAT91500.010.81971735
Produced by FastQC (version 0.11.5)