FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n01_P3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n01_P3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28906120
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC449980.15566945684858433TruSeq Adapter, Index 3 (100% over 51bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA323650.11196590894938512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG98250.035.19632
ACGTCTG103950.035.1916615
CGTCTGA107750.033.65912216
TCACTTA107700.032.12860530
CTCGTAT79650.031.44771842
CTTAGGC111350.031.39883633
AGTCACT113500.030.70452128
TTAGGCA114200.030.38014234
GTCACTT115100.030.37574629
CACTTAG117600.029.76821131
TCGGAAG124650.029.6569063
ATCGGAA124550.029.572842
CACGTCT125100.029.40384914
CACACGT127450.029.02040512
CAGTCAC124200.028.3492627
TCGTATG86250.027.99853943
TAGGCAT135050.025.14053235
CGTATGC93450.024.80520844
ACACGTC150950.024.74095313
CCAGTCA144800.024.14519526
Produced by FastQC (version 0.11.5)