FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n01_P3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n01_P3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29901600
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG60050.030.94638315
CGTCTGA63050.029.65287416
CTCGTAT51150.025.56322942
ACCGATG75500.024.2265932
CACGTCT80200.023.73224614
TCGGAAG80100.023.6234933
ATCGGAA79850.023.585182
TCGTATG55050.023.01699843
CACCGAT79450.022.82382231
GATGTAT77750.022.42751935
CACACGT84700.022.25870112
CAGTCAC90000.020.4483427
TATCTCG77200.020.1420939
CGTATGC61850.019.72198744
GTATCTC89850.019.25939438
CCAGTCA104100.017.7867426
AGTCACC104750.017.7622228
ACACGTC110000.017.58915713
TGTATCT100650.017.4834337
TCTCGTA82000.016.82401741
Produced by FastQC (version 0.11.5)