FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n01_P3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n01_P3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29071605
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG37700.023.81053715
CGTCTGA40650.022.24931716
CTCGTAT37650.018.17195542
TCGTATG44150.016.4145443
TCGGAAG56650.016.1251643
ATCGGAA57800.015.6100222
CACGTCT59650.015.46361714
CACACGT61900.014.79249612
CGTATGC51800.013.59923244
CAGTCAC67200.013.559195527
TCACGAT65600.013.2735635
AGTCACA71250.012.56738328
GTCACAT70800.012.52021729
ACACGTC77850.012.13747613
TATAGGC62400.011.93415327
ATCACGA78900.011.26402634
TCTCGTA67300.010.80137541
CCAGTCA85800.010.5410726
CATCACG87250.010.36599533
GATCGGA89850.010.29369451
Produced by FastQC (version 0.11.5)