FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14092570
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTCG7553.715286E-89.8649855
GGCCGGC5550.0089916897.5408828
GTAGAGC8550.00130333876.53380783
CCGTATC51050.05.919798494-95
CGGGGGT13453.2961638E-55.884073
GTATCAT40750.05.87613992-93
TGGTCTC9550.00456139635.8432386
CACTCTT16152.6172183E-65.7650634
GCCGTAT50600.05.696793694-95
GGGGGTC14006.0702587E-55.65284974
CGTATCA44300.05.510208692-93
CGCCGTA56200.05.33624692-93
TATCATT51400.05.06540594-95
Produced by FastQC (version 0.11.5)