FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n02_QG2990.2mGF.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n02_QG2990.2mGF.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16455921
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA192900.07.266824294-95
TCATTAA210750.06.90504394-95
ATCATTA245500.06.505263394-95
GCCGTAT275250.06.503138594-95
GTATCAT248200.06.378394-95
CGTATCA255750.06.272117692-93
CGCCGTA291500.06.0688494-95
CCGTATC292250.05.76690994-95
GGTCGCC389950.05.52624994-95
TGGTCGC400600.05.501194594-95
ATTAAAA246200.05.467013494-95
TATCATT315500.05.290353394-95
GTGGTCG448800.05.0968594-95
Produced by FastQC (version 0.11.5)