FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n02_QG2987.mGF.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n02_QG2987.mGF.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17452339
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA249450.07.418013694-95
TCATTAA276650.07.34410494-95
GTATCAT318400.06.651251394-95
GCCGTAT376650.06.597779394-95
CGTATCA338450.06.387728794-95
CGCCGTA393850.06.23881694-95
CCGTATC389500.06.212999394-95
ATCATTA312150.06.17375394-95
GGTCGCC537700.05.83216794-95
TGGTCGC550950.05.675029394-95
ATTAAAA318200.05.647254594-95
GTCGCCG553850.05.47322794-95
TCGCCGT482750.05.23471192-93
TCGGTGG779250.05.110207694-95
GTGGTCG606450.05.109996392-93
GGTGGTC647650.05.09330994-95
TATCATT400950.05.044457492-93
CGGTGGT673200.05.017409394-95