FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n02_FounderMale.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n02_FounderMale.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13333740
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA195450.07.457494-95
TCATTAA221000.07.100142594-95
ATCATTA245100.06.60117894-95
GCCGTAT294900.06.526962394-95
CGTATCA266000.06.43208494-95
GTATCAT250600.06.372813794-95
CGCCGTA309150.06.331380494-95
CCGTATC301850.05.93000594-95
ATTAAAA242850.05.89655294-95
GGTCGCC413850.05.67889494-95
TGGTCGC424550.05.442683794-95
TCGCCGT372300.05.226625492-93
GTCGCCG421700.05.12115294-95
CGGTGGG55300.05.04442594-95
TTAAAAA275900.05.03854494-95
TATCATT309750.05.0357894-95