FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n02_FounderF.B1.a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n02_FounderF.B1.a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12698409
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGGG33450.09.036059
TCTCGGG47300.07.519662494-95
GGGGCCC11550.07.448505446-47
GGAGAGG48150.06.85691268
AAAAAAA1258300.06.771078694-95
GGGGGGC47100.06.269045444-45
GGGAGAG51550.06.22427377
GGGCCCG9655.5983037E-85.78273546-47
TATCATT430800.05.76319994-95
GTATCAT408650.05.728567694-95
TCGGGAG30200.05.7031575
CATTAAA418250.05.663779394-95
GGGTGGG33250.05.66362918-19
ATCATTA447850.05.626842594-95
TCATTAA448550.05.519589494-95
CGTATCA448350.05.47048292-93
ATTAAAA427200.05.419961594-95
TTAAAAA451250.05.22897994-95
GGGGGAA65250.05.202112722-23
GAGGGGC23750.05.18832810-11
Produced by FastQC (version 0.11.5)