FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n02_FounderF.A1.b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n02_FounderF.A1.b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15833733
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGGG51550.08.7500229
GGGGCCC16600.08.26402646-47
GGAGAGG64250.07.23759568
GGGGGGC69000.06.57065344-45
TCTCGGG70150.06.39573194-95
GAGGGGC31650.05.87672310-11
AAAAAAA2233550.05.86799894-95
GGGGGAA85000.05.55251622-23
GGGAGAG77550.05.4566797
GGGCCCC14754.0017767E-115.359627746-47
TCGGGAG40200.05.21068675
AAAGGGG68650.05.113847328-29
AGGGGCG59300.05.096667312-13
CATTAAA663350.05.088437694-95
TCATTAA706300.05.01598694-95