FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14092570
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCTAG5400.029.9640322-23
GCTAGTG6450.025.98209824-25
CTTAGGT3800.025.85242324-25
GCTTAGT6700.024.58136422-23
GCTAGGT3650.023.74839824-25
CCTAGCG2800.023.73426424-25
CTAGCGT3300.023.64045326-27
GCTAGCG4850.023.23425924-25
AGCTAGG4500.022.47302224-25
CTAGCGA5200.022.22606724-25
ACCCTAG3150.022.01438922-23
TGCTAGT7100.021.97563222-23
CTAGTGC6200.021.90342924-25
AGCCTAG3700.021.86564322-23
CCTATAG4900.021.81783324-25
TTGCTAG8700.021.58738722-23
AGGCTAG5050.021.16977722-23
GCTAGCA5350.021.06283224-25
CGCTAGG3300.021.01373524-25
TATCTAG5800.020.9231622-23
Produced by FastQC (version 0.11.5)