FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n01_QG2990.2mGF.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n01_QG2990.2mGF.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16455921
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGTC19500.07.109423624-25
CTGCTTG241500.06.264417694-95
TGCTTGA218600.05.92434494-95
GTATGCC427100.05.762864694-95
TATGCCG401100.05.698525494-95
CTCTTAG25500.05.66314422-23
CACTTAG21600.05.61595122-23
ATGCCGT394250.05.57775294-95
CGTATGC455100.05.562680794-95
TGCCGTC400650.05.50034394-95
GCCGTCT386650.05.49356994-95
GCTTGAA208350.05.48518994-95
TCGTATG492800.05.322462694-95
CTTAGAC15651.9184881E-85.167392724-25
CCTAGCG10101.0550332E-45.147293624-25
CTCGTAT533850.05.141308394-95
TCTGCTT324950.05.13852394-95