FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n01_QG2990.2fGM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n01_QG2990.2fGM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18034862
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGA2350.019.65294624-25
GCTAGGT7200.016.03625924-25
GCTAGAC5950.015.52417724-25
CCTAGGT2701.9172148E-914.96717524-25
CTAGGTG5650.014.81580126-27
TCTAGCA8950.014.51326224-25
GCTAGGC8900.014.27046824-25
CTAGGTA3452.0008883E-1114.22346326-27
TCCTAGG2451.4216494E-714.13808924-25
TCTAGCT7050.013.92083824-25
CTAGGCC4800.013.83127324-25
ACTAGTG7900.013.51917624-25
GTCTAGC6650.013.45598824-25
CTAGGGT3251.8135324E-913.32243124-25
CTCTAGC5450.013.24094824-25
GCTAGAA12700.013.18256224-25
ACCCTAG5050.013.14655722-23
CCTAGAC4400.013.12057624-25
CCTAGGC3302.2773747E-913.12057524-25
CCTAGCG9050.013.07708224-25
Produced by FastQC (version 0.11.5)