FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n01_QG2990.1mGF.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n01_QG2990.1mGF.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14573299
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT291250.06.08722794-95
TATGCCG294400.06.006184694-95
CTGCTTG178000.05.960294794-95
ACTTAGC12705.1986717E-95.90756522-23
CGTATGC340050.05.840494-95
CACTTAG18800.05.83263322-23
GTATGCC318500.05.831137794-95
TGCTTGA170000.05.827482794-95
TGCCGTC297500.05.82551594-95
CTTAGCC12502.2706445E-85.77123724-25
CTCGTAT393650.05.717453594-95
TCGTATG370450.05.63297394-95
CTCTTAG22600.05.49033622-23
TCTCGTA440850.05.471871494-95
GCCGTCT293350.05.4368994-95
GACTTAG13751.7884668E-75.24657922-23
CTTAGCA23900.05.19169824-25
CTTAGTA10654.4731554E-55.148051324-25
CCGTCTT315200.05.134301794-95
ATCTTAG20951.4551915E-115.096318722-23