FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n01_QG2990.1fGM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n01_QG2990.1fGM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17633222
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA272700.06.59557394-95
CTGCTTG296300.06.41011394-95
GCTTGAA258150.06.114546394-95
CGCTAGC15651.0186341E-105.718913622-23
GCCGTCT493400.05.705343294-95
GTATGCC573400.05.632264694-95
TCTGCTT377100.05.558312494-95
GCTAGCG15656.039045E-105.534461526-27
TGCCGTC523000.05.44065494-95
CGTATGC603550.05.420752594-95
TATGCCG544400.05.40316294-95
ATGCCGT526750.05.36635294-95
CTAGCGC15703.758032E-95.332941526-27
GTCTTAG17951.2914825E-105.307813622-23
CCGTCTT503400.05.266349394-95
CTCGTAT674000.05.06957894-95
TCGTATG638900.05.069507694-95
CCGCTAG18351.2860255E-95.034775322-23