FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n01_FounderMale.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n01_FounderMale.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13333740
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG220100.06.91616994-95
TGCTTGA204500.06.676479394-95
CTTAGCC13551.4551915E-116.394022524-25
GCTTGAA195050.06.327569594-95
GCCGTCT384200.06.065194-95
TGCCGTC398450.05.96574294-95
TCTTAGT22300.05.95724122-23
GTATGCC442450.05.817089694-95
TATGCCG414350.05.770728694-95
ATGCCGT402450.05.755041692-93
TCTGCTT290500.05.747985494-95
CGTATGC471950.05.572576594-95
TCCTTAG19701.8189894E-125.424100422-23
CTTAGTC16001.0568328E-95.41493824-25
TCTTAGC21350.05.41071122-23
CCGTCTT389850.05.38849194-95
CTTCTGC380200.05.29734994-95
CTTAGCA19651.0913936E-115.29093224-25
CTTAGTG15401.3093086E-85.250849224-25
CACTTAG16502.3246685E-95.250849222-23
Produced by FastQC (version 0.11.5)