FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n01_FounderF.B1.b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n01_FounderF.B1.b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11393874
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT2000.023.08865524-25
GTCCTAG2053.8198777E-1119.7098322-23
CTAGGTA2458.598363E-915.31390426-27
CGCCTAG4500.014.75108522-23
ACTAGGC3950.014.61307424-25
CTAGGCG4850.014.28164326-27
CCTAGCG5200.013.87539424-25
GCCTAGG2501.8225728E-713.85319222-23
TGCCTAG5500.013.64329722-23
GGCCTAG4101.8189894E-1213.37452722-23
ACCTAGC4001.8189894E-1112.98736924-25
CTCTAGC4551.8189894E-1212.68607524-25
CCTAGCC4255.2750693E-1112.22340724-25
GTCTAGT5450.012.17979524-25
GACTAGG2655.2738815E-611.97996324-25
TGCTAGG4853.6379788E-1211.90136924-25
GCCCTAG3902.1518645E-911.84033622-23
CTAGAGC4551.72804E-1011.41746724-25
CTAGGCA4308.1854523E-1011.4100926-27
CCTAGTC3304.2312604E-711.36941324-25