FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n01_FounderF.B1.a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n01_FounderF.B1.a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12698409
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTA3200.017.13701226-27
CCTAGGC2458.592906E-915.31471124-25
CTCTAGT6250.015.23931424-25
CCTAGGT1200.004857235614.431169524-25
ACCTAGG2003.8517974E-614.43116922-23
GCTAGAC5000.014.43116924-25
CTAGTCA7450.014.33431526-27
ACTAGTC4900.014.13665624-25
TGCTAGG4001.8189894E-1213.7096124-25
CTCTAGC4900.013.54762824-25
CTAGAGT4051.8189894E-1213.54035624-25
CCTAGCC4051.8189894E-1213.54035624-25
CCTAGTC3008.458301E-913.46909124-25
GCTAGAG6250.013.39212524-25
ACTCTAG5400.013.36219422-23
GCTAGGT4153.45608E-1112.51860524-25
TACCTAG3252.591878E-812.43300722-23
TCTAGCG7450.012.39724524-25
TCTAGCA8350.012.09798624-25
CTAGCTG9100.012.05240426-27
Produced by FastQC (version 0.11.5)