FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l08_n01_FounderF.A1.a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l08_n01_FounderF.A1.a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11743953
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGC2852.4920155E-1015.20782224-25
GCCTAGC5250.014.30983524-25
CCTAGCC4500.014.12637624-25
TCTAGTG7550.013.39496824-25
CGCCTAG6400.013.09298422-23
CCTAGCA5300.013.08446524-25
CTAGGGT3101.3182216E-813.04929326-27
TCTAGCA8000.013.00268724-25
CTAGGTA2959.705582E-812.73332926-27
ACCTAGC5250.012.658724-25
GCTAGAC5600.012.38351224-25
GGCTAGG4803.6379788E-1212.03952524-25
CTAGCTG9750.011.85430126-27
TGCTAGG4451.1459633E-1011.68780924-25
CTCTAGC4451.1459633E-1011.68780922-23
GCTAGGG4702.3646862E-1111.68090224-25
ATCTAGC9350.011.4343324-25
GGCCTAG4359.731593E-1011.29224522-23
GCTAGGC5900.011.26409824-25
ATCCTAG6450.011.19955822-23