FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12549715
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN181190.1443777806906372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTGC3800.030.6411383
GATCCAG5200.028.6612513
GATCCGC3450.025.649743
GATCCGG3350.023.6348363
GATCACG3750.023.5977613
GATCCTG5750.023.4897613
GATCGGC3600.023.2872643
GATCTAG4200.022.1783493
GATCCTC7300.020.4162313
GATCCGT3252.910383E-1120.0628743
GATCTCT7800.019.1074983
GATCTGG4400.019.0532153
GATCGAC5000.018.6298123
GATCTGT5350.018.2815913
GATCGTG4850.018.2456933
GATCAAG9200.018.2248153
GATCAGG4650.018.028853
GATCGGT3107.1413524E-918.0288493
GATCCAC6000.017.853573
GATCACC4450.017.7925173
Produced by FastQC (version 0.11.5)