FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n02_DJ58_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n02_DJ58_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27544950
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC49850.028.8709073
GATCACG78050.026.1974323
GATCGCG36200.025.218233
GATCCCG61150.024.8306583
GATCCGG60250.023.9646853
GATCTGC99100.023.8287583
GATCCAG117950.023.4166013
GATCAGC108500.023.052163
GATCCGT84050.022.8310873
GATCCTT243700.022.1319733
GATCCTG102700.022.0864333
GATCCGA115800.021.880513
GATCGTG99700.021.8166893
GATCCAC134950.021.32963
GATCCTC147050.021.0314963
GATCTCG98550.020.8424643
GATCAAC198350.020.7345983
GATCTTG152450.020.62263
GATCACA212650.019.7783243
GATCACC94050.019.6606943
Produced by FastQC (version 0.11.5)