FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n02_DJ57_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n02_DJ57_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23845545
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG26450.023.421393
GATCCGC39250.023.2596473
GATCCGG47550.022.1383193
GATCTGC74750.020.5008283
GATCACG61150.019.6521453
GATCCAG91000.018.6826383
GATCCAC97450.018.497623
GATCCCG49350.018.3105473
GATCTGG69500.018.2293343
GATCCGA92300.018.1167143
GATCAGC88450.017.8520723
GATCCTG77800.017.1227363
GATCTCG81200.017.094133
GATCCTT199800.017.041533
GATCGCC45100.016.9376853
GATCCTC114900.016.296443
GATCCGT66350.016.2165243
GATCGTG80300.016.0675983
GATCTTG119200.015.9039463
GATCTCC92650.015.7859573
Produced by FastQC (version 0.11.5)