FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n02_DJ56_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n02_DJ56_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32686192
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC72600.035.606873
GATCCGG84450.032.596073
GATCGCG48300.029.5088083
GATCTGC129350.029.4913623
GATCCGA160850.028.580743
GATCCAG163050.028.4236353
GATCCCG78350.028.1784273
GATCACG102600.028.1463263
GATCCTG135000.027.8085693
GATCCGT113450.027.7536143
GATCAGC145350.027.0781543
GATCTCG134600.026.6454493
GATCCAC183550.026.391033
GATCTGG125800.025.76953
GATCCTC193450.025.7627623
GATCTCC164900.025.4213893
GATCGGG67300.024.569183
GATCTTG213700.024.5420743
GATCACC128700.024.4288273
GATCCTT329400.024.4200273
Produced by FastQC (version 0.11.5)