FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n02_DJ55_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n02_DJ55_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34423931
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC61700.034.8001373
GATCCGG69550.034.2207073
GATCCCG79750.029.7854883
GATCTGC118500.029.2035523
GATCCAG135950.028.881093
GATCACG89850.028.2516383
GATCAGC128400.027.3508953
GATCCGA131050.026.9044463
GATCTCG121450.026.499993
GATCCTG124500.026.3745463
GATCGCG43100.025.8277053
GATCCGT103800.025.441983
GATCTGG106450.025.2461643
GATCGCC72150.023.9498483
GATCGGG62500.023.8470783
GATCTTG185500.023.275613
GATCCTC173500.023.274743
GATCGTG124500.023.2694573
GATCCAC156050.023.1314663
GATCTCC143050.022.759073
Produced by FastQC (version 0.11.5)