FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n02_DJ54A_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n02_DJ54A_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24970687
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC42450.030.5032063
GATCCGG47300.028.3602283
GATCACG66150.026.33423
GATCCCG52200.026.0549773
GATCTGC75750.025.3948553
GATCCAG92400.024.901953
GATCGCG31050.024.3014283
GATCTGG75800.023.5961113
GATCCGA92150.023.352053
GATCTCG88950.022.8306833
GATCCTG86400.022.5341383
GATCCAC107100.022.2668553
GATCTCC100550.021.9570833
GATCCGT72850.021.9302183
GATCCTT199850.021.7215163
GATCCTC121900.021.3210643
GATCAGC90650.021.1693633
GATCTTG124300.021.0967543
GATCGCC48300.020.733363
GATCGTG81550.020.0475623
Produced by FastQC (version 0.11.5)