FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n02_DJ53_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n02_DJ53_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25587138
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC62350.033.691013
GATCCGG65950.030.4394233
GATCACG85850.028.5377253
GATCCCG66900.028.2666173
GATCCAG137950.027.9564843
GATCCGA123900.027.7809493
GATCGCG39150.027.4823683
GATCCAC148200.027.280073
GATCCTG107750.027.189883
GATCTGC104100.027.0693973
GATCCGT92200.025.4103723
GATCAGC116600.024.846533
GATCCTC154500.024.8412063
GATCGTG103450.024.4029733
GATCTCG108150.023.9023383
GATCAAC223450.023.887913
GATCGGG54650.023.6082363
GATCCCA111350.023.3409183
GATCACA217300.023.1921753
GATCAAG221400.022.9941043
Produced by FastQC (version 0.11.5)