FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12549715
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGC1007.2941475E-1032.477626-27
GATCCGG2800.031.4542271
CAGATCT2800.030.15776826-27
GATCCAC6050.028.3484821
GGATCTC1900.027.34955626-27
AGAGTCC3050.026.62098326-27
AGATCCT2200.025.09632726-27
CCTGAGG3000.023.81590822-23
TAGCTGG7200.023.45604326-27
AGATCTG3200.023.34327326-27
TAGCCCT2650.023.28582426-27
AGCGCTG3950.023.02209526-27
GATCTCC5450.022.964171
GATCTTC12750.022.9041331
ACTAGCC3550.022.87135724-25
GCTAGTG4700.022.80322824-25
GGTCGAC4850.022.76684622-23
TAGCGCT4350.022.39834226-27
CGACCTG4100.022.17982326-27
TGGCGCG5150.022.07122822-23