FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n01_DJ58_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n01_DJ58_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27544950
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGG62100.025.9228481
GATCCGC51950.024.6472431
GATCACG81450.023.8653471
GATCCGA114050.023.6740471
GATCCTG106750.023.2069591
GATCCAG122800.023.044981
GATCCGT86050.022.9130481
GATCTGC93200.022.1507741
GATCCAC124350.022.1234951
GATCCTT241550.021.760441
GATCGCG36600.021.6750371
GATCTCG103900.021.164481
GATCCTC150150.021.1337031
GATCTTG153150.021.0832251
GATCGTG95900.021.0433791
GATCCCG59250.020.9841441
GATCAAC194600.020.6929171
GATCAGC108400.019.7723141
GATCTTC282100.019.553461
GATCTCC122550.019.533551
Produced by FastQC (version 0.11.5)