FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n01_DJ57_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n01_DJ57_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23845545
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC39100.024.3110641
GATCCGG44150.023.1056841
GATCGCG31200.021.1037981
GATCCAG91700.019.9229561
GATCCTG78450.019.3277661
GATCTGC73300.019.2940221
GATCTGG68650.018.7772141
GATCACG63100.018.5916541
GATCCGA89800.018.4856011
GATCCCG48950.018.2823281
GATCTCG81200.017.2455791
GATCCTT194200.017.1197261
GATCCAC101100.017.1074221
GATCCGT68250.017.0528891
GATCGCC43950.016.8806041
GATCAGC86400.016.7443281
GATCCTC118150.016.4832341
GATCGGG42050.016.099551
GATCGTG73850.016.0109311
GATCTTG118700.015.4693251
Produced by FastQC (version 0.11.5)