FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n01_DJ56_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n01_DJ56_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32686192
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC75450.038.0063551
GATCCGG81750.032.1826861
GATCTGC137300.031.1592771
GATCCCG79500.029.2997251
GATCCGT117400.028.6152421
GATCCTG139200.028.3672521
GATCGCG46800.027.761261
GATCCAG161700.027.7200551
GATCCAC180750.027.6223721
GATCCGA156600.027.4672361
GATCTCG139950.027.3200361
GATCACG107250.027.2132451
GATCAGC141600.026.9361521
GATCCTC191600.026.130811
GATCTCC168000.026.0453011
GATCTTG217800.024.9048331
GATCCCC72600.024.8622231
GATCCCA141100.024.3350181
GATCCTT318850.024.2882881
GATCGTG128650.023.696411
Produced by FastQC (version 0.11.5)