FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n01_DJ55_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n01_DJ55_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34423931
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGG69200.035.333411
GATCCGC62850.033.588231
GATCTGC110950.032.700961
GATCCAG137850.029.4834521
GATCACG96300.029.099911
GATCCTG121000.028.2977581
GATCCCG73350.027.5782931
GATCCGT99900.027.3546281
GATCTGG99050.026.7462331
GATCGCG42700.026.4033741
GATCCGA133800.026.3881871
GATCCAC155700.026.1629621
GATCAGC124950.025.8436581
GATCTCG119850.024.775531
GATCGGC88450.024.0241831
GATCTTG185850.023.8907831
GATCGTG118850.023.8519041
GATCGCC65850.023.7440681
GATCCTT280250.023.177331
GATCCCA125900.022.9584921
Produced by FastQC (version 0.11.5)