FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n01_DJ54A_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n01_DJ54A_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24970687
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC42900.032.530921
GATCCGG47350.029.7673871
GATCTGC80100.025.7581481
GATCGCG31450.025.6516761
GATCCGA89150.025.5356711
GATCCAG99650.025.217911
GATCCTG81200.024.2101211
GATCCCG50350.023.7578621
GATCACG69900.023.4808181
GATCTCG83150.023.0289921
GATCCAC105400.022.742441
GATCCGT69900.022.4195381
GATCAGC87550.022.3482461
GATCGTG81400.022.1001111
GATCCTC122250.021.7695851
GATCACC76250.021.3429451
GATCCTT200900.021.2783581
GATCGCC44650.020.4565261
GATCTCC102150.020.1980191
GATCTGG71400.019.8056341
Produced by FastQC (version 0.11.5)