FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l07_n01_DJ53_demultiplex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l07_n01_DJ53_demultiplex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25587138
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC59900.030.4324721
GATCACG87450.028.2708531
GATCCTG108900.028.0265451
GATCGCG37650.027.9661331
GATCCGG66850.027.6849041
GATCCCG67700.027.200281
GATCTGC105900.027.112291
GATCCAG127250.026.7552991
GATCCAC143450.025.9648911
GATCCGA116450.025.9306281
GATCGTG102000.025.647821
GATCCTT253000.025.025551
GATCCGT88450.024.5949821
GATCACC100350.024.544191
GATCAAC219550.024.3594441
GATCCTC158100.024.0576651
GATCAGC120650.023.989921
GATCTGG99550.023.7629551
GATCCCA109000.023.5751691
GATCTCG106600.023.3227141
Produced by FastQC (version 0.11.5)