FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16642421
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT6350.018.8628868
CGGTTAG4303.6379788E-1217.8276777
GACAGTC5107.8216544E-1014.0916429
TAGACTG6551.8189894E-1213.8981037
AGTACCG8000.013.7746667
GTCTATG8800.013.7314663
GTCCTAT10900.013.2178693
CCTATAC11700.013.1071175
GTTAGCG3701.1801054E-512.9490779
GGACCGT6301.0186341E-1012.9285628
TATCCTG11550.012.8594667
TACCGTG7103.6379788E-1212.821419
CGCATAG3801.5524884E-512.608397
GATAGTC6853.092282E-1112.5899069
GCCAGTA6251.2350938E-912.2653669
AGACACT8300.012.1222468
GAGAGTC6453.6379788E-1211.8850459
AATCCCG8500.011.8370537
TGTATAC8250.011.8289035
TCTGGAC6704.7475623E-1011.7910715
Produced by FastQC (version 0.11.5)