FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_SAL60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_SAL60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13671613
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG45100.017.3138283
CCGTATC183100.015.4794694-95
CCTAGAC48500.014.4694655
TATACCG51100.014.4061677
TAGACGG18650.014.3535017
CGTATCA165700.013.70355194-95
GTCCTGG32850.013.4415263
GACTGTG21400.013.4024099
GTCTATG55600.013.2917653
CTAGACA62600.013.1936196
TAGAGGT56650.013.0231326
GTACCGT33950.012.8129368
GTATAAG60500.012.753023
GTGTATG68200.012.471743
GACTGCG16500.012.4574719
GTATCAT159800.012.380794-95
CATAGAC57000.012.2302055
TAGACCG28350.012.1402637
TACACAG39550.012.0865467
GTATAGG62500.011.9730883
Produced by FastQC (version 0.11.5)