FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_SAL60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_SAL60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8331848
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG29150.016.74123
TATACCG26450.016.2810787
CCTAGAC30750.015.5662325
CTAGACA40950.014.04952056
GTCTAGA30750.013.9052423
GTACCGT19300.013.6355218
TAGACGG11950.013.6137377
CTAAGGT32500.013.4195596
TAGAGGT31200.013.3838696
AGTACCG24250.013.2199387
TAGAGCG27450.013.0733017
GTCTATG32950.012.9768183
CTATACT42000.012.92499356
TATACTG37100.012.8971117
ATACTGC31400.012.8001788
TCTATAC33800.012.78667455
TAGACAC31150.012.7493017
TGGTCGC307700.012.73219492-93
CGCCGTA172450.012.61496194-95
GACTGTG16950.012.4207939
Produced by FastQC (version 0.11.5)