FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_SAL5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_SAL5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18563581
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC200950.015.4540594-95
GCCGTAT250450.014.48323194-95
CGCCGTA299850.012.73138194-95
CGTATCA159650.012.3916794-95
TGGTCGC570050.011.05149592-93
GGTCGCC580150.010.92169894-95
TCTCGGG101250.010.81176894-95
TATACTG93700.010.1085287
TATACCG49200.09.6257037
GTATCAT184250.09.61691294-95
TCGCCGT478150.09.58456894-95
GTCGCCG704450.09.52133694-95
GTGGTCG636350.09.42620192-93
GGTGGTC685650.09.27963890-91
TAGACAG58200.09.2057117
GTATTAG79550.09.114053
GTATTGG99250.08.8034783
TACACAG67500.08.7878027
GTCTAGG61900.08.7846033
TCGGTGG812100.08.54593988-89
Produced by FastQC (version 0.11.5)